Coding
Part:BBa_K4258020:Design
Designed by: Jake Schumacher Group: iGEM22_NDSU (2022-09-26)
mScarlet-His_NegControl
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 16
Illegal BsaI.rc site found at 746
Design Notes
Bpi1 and Bsa1 sites added to the ends for Golden Gate Cloning.
Source
mScarlet from Addgene Plasmid #121003